Fast, easy, and efficient, the phosphatase-free, ligation-free Cold Fusion Cloning Kit will take you to transformation-ready DNA in 20 minutes and a single step. SBI’s Cold Fusion Cloning is an excellent choice for any cloning project. Whether you're assembling multiple fragments of DNA or simply adding an insert or gBlock to a vector, the Cold Fusion Cloning Kit will take you to transformation-ready DNA in a single step—just incubate your DNA fragment(s) with linearized vector for five minutes at room temperature and ten minutes on ice. A typical cloning reaction will deliver a successful cloning rate of more than 90%.